Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- PA5-53775 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ataxin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ataxin 2 in U2OS cells transfected with control siRNA, target specific siRNA probe #1, using a Ataxin 2 Polyclonal Antibody (Product # PA5-53775). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ataxin 2 in human cell line K562 using a Ataxin 2 Polyclonal Antibody (Product # PA5-53775).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ataxin 2 in mouse cell line NIH-3T3 using a Ataxin 2 Polyclonal Antibody (Product # PA5-53775).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Ataxin 2 in human cell line U-251 MG shows positivity in cytoplasm. Samples were probed using an Ataxin 2 Polyclonal Antibody (Product # PA5-53775).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human testis and skeletal muscle tissues using Ataxin 2 Polyclonal Antibody (Product # PA5-53775). Corresponding ATXN2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human colon, kidney, skeletal muscle and testis using Ataxin 2 Polyclonal Antibody (Product # PA5-53775) (A) shows similar protein distribution across tissues to an independent Ataxin 2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human skeletal muscle using Ataxin 2 Polyclonal Antibody (Product # PA5-53775) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human testis using Ataxin 2 Polyclonal Antibody (Product # PA5-53775) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human colon using Ataxin 2 Polyclonal Antibody (Product # PA5-53775).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human kidney using Ataxin 2 Polyclonal Antibody (Product # PA5-53775).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Ataxin-2 was performed on HAP1 cell lysates. Antibody-bead conjugates were prepared by adding 1 µg of Ataxin polyclonal antibody (Product # PA5-53775) with 30 µL of protein A -Sepharose beads and rocked overnight at 4 deg. 1 µg of lysate was incubated with antibody-bead conjugate for 2 hrs at 4 deg. After multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different ATXN polyclonal antibody (Product # PA5-54183). Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.