Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
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Validation data
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- Product number
- PA5-54336 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Ataxin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RHPRNHRVSA GRGSISSGLE FVSHNPPSEA ATPPVARTSP SGGTWSSVVS GVPRLSPKTH RPRSPRQNSI GNTPSGPVLA SPQAGIIPTE AVAMPIPAAS PTPASPASNR AVTPSSEAKD SRLQDQRQNS PAGNKENIKP NET Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 91%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ataxin 2 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an Ataxin 2 Polyclonal Antibody (Product # PA5-54336).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Ataxin 2 in human cell line U-2 OS shows positivity in nucleoli & cytoplasm. Samples were probed using an Ataxin 2 Polyclonal Antibody (Product # PA5-54336).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human cerebral cortex tissue shows strong cytoplasmic positivity in neuronal cells. Samples were probed using an Ataxin 2 Polyclonal Antibody (Product # PA5-54336).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human testis and skeletal muscle tissues using Ataxin 2 Polyclonal Antibody (Product # PA5-54336). Corresponding ATXN2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human colon, kidney, skeletal muscle and testis using Ataxin 2 Polyclonal Antibody (Product # PA5-54336) (A) shows similar protein distribution across tissues to an independent Ataxin 2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human skeletal muscle using Ataxin 2 Polyclonal Antibody (Product # PA5-54336) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human testis using Ataxin 2 Polyclonal Antibody (Product # PA5-54336) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human kidney using Ataxin 2 Polyclonal Antibody (Product # PA5-54336).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ataxin 2 in human colon using Ataxin 2 Polyclonal Antibody (Product # PA5-54336).