Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [4]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 21776-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ataxin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 21776-1-AP (ATXN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 21776-1-AP ( Ataxin 2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21776-1-AP (ATXN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- DU 145 cells were subjected to SDS PAGE followed by western blot with 21776-1-AP (ATXN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21776-1-AP (ATXN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 21776-1-AP (Ataxin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 21776-1-AP (Ataxin 2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 21776-1-AP (Ataxin 2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using ATXN2 antibody 21776-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 21776-1-AP (Ataxin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 21776-1-AP (Ataxin 2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas using 21776-1-AP (ATXN2 antibody) at dilution of 1:25 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas using 21776-1-AP (ATXN2 antibody) at dilution of 1:25 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 21776-1-AP (Ataxin 2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Ataxin 2 (IP:21776-1-AP, 4ug; Detection:21776-1-AP 1:1000) with HEK-293 cells lysate 1200ug.