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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 12170-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RTF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MKKQANKTA SSGSSDKDSS AESSAPEEGE VSDSDSNSSS SSSDSDSSSE DEEFHDGYGE DLMGDEEDRA RLEQMTEKER EQELFNRIEK REVLKRRFEI KKKLKTAKKK EKKEKKKKQE EEQEKKKLTQ IQESQVTSHN KERRSKRDEK LDKKSQAMEE LKAEREKRKN RTAELLAKKQ PLKTSEVYSD DEEEEEDDKS SEKSDRSSRT SSSDEEEEKE EIPPKSQPVS LPEELNRVRL SRHKLERWCH MPFFAKTVTG CFVRIGIGNH NSKPVYRVAE ITGVVETAKV YQLGGTRTNK GLQLRHGN (1-307 aa encoded by BC015052)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HL-60 cells were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP ( RTF1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP ( RTF1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RTF1 (IP:12170-1-AP, 4ug; Detection:12170-1-AP 1:500) with HeLa cells lysate 2400ug.
