NB600-278

antibody from Novus Biologicals

Targeting: RTF1 KIAA0252

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB600-278

Provider

Novus Biologicals

Proper citation

Novus Cat#NB600-278, RRID:AB_10000546

Product name

Rabbit Polyclonal RTF1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RTF1 Antibody [NB600-278] - Detection of Human Rtf1 on HeLa whole cell lysate using NB600-278. Rtf1 was IPed with anti-Rtf1 antibodies NB600-277 or NB600-278 using 3 mcg/10 mg lysate. IPed Rtf1 was blotted using NB600-277.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RTF1 Antibody [NB600-278] - Detection of Human Rtf1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rtf1 antibody NB600-278 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: RTF1 Antibody [NB600-278] - Detection of human Rtf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rtf1 antibody NB600-278 used for IP at 3 ug per reaction. Rtf1 was also immunoprecipitated by rabbit anti-RTF1 antibodies NB600-277 and NB100-61052. For blotting immunoprecipitated Rtf1, NB600-278 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.