Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB600-277 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-277, RRID:AB_2185959
- Product name
- Rabbit Polyclonal RTF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RTF1 Antibody [NB600-277] - Detection of Human Rtf1 on HeLa whole cell lysate using NB600-277. Rtf1 was immunoprecipitated with NB600-277 or NB600-278 using 3 mcg/10 mg lysate. IPed Rtf1 was blotted using NB600-277 at 0.1 mcg/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RTF1 Antibody [NB600-277] - Detection of human RTF1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RTF1 antibody NB600-277 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RTF1 was successfully immunoprecipitated by rabbit anti-RTF1 antibodies NB600-278 and NB100-61052, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).