Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- R1476P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1476P, RRID:AB_973120
- Product name
- anti BRIP1 / FANCJ
- Antibody type
- Polyclonal
- Antigen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 92-104 of isoform 1 of human BACH1 protein.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Western blot using Affinity Purifiedanti-BACH1 antibody shows detection of a band at~105 kDa (lane 1) corresponding to human BACH1present in a 293 whole cell lysate (arrowhead). Lane 2shows that specific band staining is competed out whenthe antibody is pre-incubated with the peptideimmunogen prior to reaction. Approximately 35 µg oflysate was separated on a 4-20% Tris-Glycine gel bySDS-PAGE and transferred onto nitrocellulose. Afterblocking the membrane was probed with the primaryantibody diluted to 1:1,000. Reaction occurred 2 h atroom temperature followed by washes and reactionwith a 1:10,000 dilution of IRDye TM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at roomtemperature (800 nm channel, green). Molecularweight estimation was made by comparison toprestained MW markers in lane M (700 nm channel,red). IRDye TM800 fluorescence image was capturedusing the Odyssey® Infrared Imaging Systemdeveloped by LI-COR. IRDye is a trademark of LI-COR,Inc. Other detection systems will yield similar results.