A300-561A

antibody from Invitrogen Antibodies

Targeting: BRIP1 BACH1, FANCJ, OF

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-561A

Provider

Invitrogen Antibodies

Product name

BRIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRIP1/BACH1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-BRIP1/BACH1 antibody (Product # A300-561A; lot A300-561A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRIP1(BACH1) by western blot and immunoprecipitation. expndtw-6 Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP) and 293T (T; 50 µg for WB) cells. Antibody: Affinity purified rabbit anti-BRIP1(BACH1) antibody BL2600 (Cat. No. A300-561A) used for WB at 0.04 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate. BRIP1(BACH1) was also immunoprecipitated using rabbit anti-BRIP1(BACH1) antibody BL2599 (Cat. No. A300-561A), which is to an upstream epitope, at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRIP1/BACH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-BRIP1/BACH1 antibody (Product # A300-561A; lot A300-561A-1) used for IP at 3 µg/mg lysate. BRIP1/BACH1 was also immunoprecipitated using rabbit anti-BRIP1/BACH1 antibody (Product # A300-560A), which is to an upstream epitope, at 3 µg/mg lysate. For blotting immunoprecipitated BRIP1/BACH1, Product # A300-561A was used at 1 µg/mL.Detection: Chemiluminescence with exposure time of 10 seconds.