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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-31883 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31883, RRID:AB_2066306
- Product name
- Rabbit Polyclonal BRIP1/FANCJ Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references ATR-mediated proteome remodeling is a major determinant of homologous recombination capacity in cancer cells.
FancJ (Brip1) loss-of-function allele results in spermatogonial cell depletion during embryogenesis and altered processing of crossover sites during meiotic prophase I in mice.
Kim D, Liu Y, Oberly S, Freire R, Smolka MB
Nucleic acids research 2018 Sep 19;46(16):8311-8325
Nucleic acids research 2018 Sep 19;46(16):8311-8325
FancJ (Brip1) loss-of-function allele results in spermatogonial cell depletion during embryogenesis and altered processing of crossover sites during meiotic prophase I in mice.
Sun X, Brieño-Enríquez MA, Cornelius A, Modzelewski AJ, Maley TT, Campbell-Peterson KM, Holloway JK, Cohen PE
Chromosoma 2016 Jun;125(2):237-52
Chromosoma 2016 Jun;125(2):237-52
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Sample (30 ug of whole cell lysate) A: Hela 5% SDS PAGE FANCJ antibody, antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883] - Paraffin-embedded SAS xenograft; antibody dilution 1:500.
