PA5-27096

antibody from Invitrogen Antibodies

Targeting: BRIP1 BACH1, FANCJ, OF

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27096

Provider

Invitrogen Antibodies

Product name

BRIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: HeLa. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of FANCJ showing staining in the cytoplasm of 293T cells. 293T cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using a FANCJ polyclonal antibody (Product # PA5-27096) diluted at 1:500. Blue: Hoechst 33343 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BRIP1 Polyclonal Antibody detects BRIP1 protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: BRIP1 protein stained by BRIP1 Polyclonal Antibody (Product # PA5-27096) diluted at 1:500. Blue: Hoechst 33343 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BRIP1 Polyclonal Antibody detects BRIP1 protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: BRIP1 protein stained by BRIP1 Polyclonal Antibody (Product # PA5-27096) diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BRIP1 Polyclonal Antibody detects BRIP1 protein at nucleus on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. BRIP1 Polyclonal Antibody (Product # PA5-27096) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.