MAB6496

antibody from R&D Systems

Targeting: BRIP1 BACH1, FANCJ, OF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6496

Provider

R&D Systems

Product name

Human BRIP1/FANCJ Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human BRIP1/FANCJ in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FANCA, D2, E, F, G, I, L, M, or recombinant mouse BRIP1/FANCJ is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q9BX63


Isotype

IgG

Antibody clone number

652747

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human BRIP1/FANCJ by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human BRIP1/FANCJ Monoclonal Antibody (Catalog # MAB6496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BRIP1/FANCJ at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

BRIP1/FANCJ in Human Breast Cancer Tissue. BRIP1/FANCJ was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BRIP1/FANCJ Monoclonal Antibody (Catalog # MAB6496) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hemotoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.