26583-1-AP

antibody from Invitrogen Antibodies

Targeting: LIG3 LIG2, LIG3alpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

26583-1-AP

Provider

Invitrogen Antibodies

Product name

LIG3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: CDPRHKDCL LREFRKLCAM VADNPSYNTK TQIIQDFLRK GSAGDGFHGD VYLTVKLLLP GVIKTVYNLN DKQIVKLFSR IFNCNPDDMA RDLEQGDVSE TIRVFFEQSK SFPPAAKSLL TIQEVDEFLL RLSKLTKE (254-390 aa encoded by BC068005)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.5 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293T cells were subjected to SDS PAGE followed by western blot with 26583-1-AP ( LIG3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293T cells were subjected to SDS PAGE followed by western blot with 26583-1-AP (LIG3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 26583-1-AP (LIG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-LIG3 (IP:26583-1-AP, 4ug; Detection:26583-1-AP 1:300) with HEK-293T cells lysate 4000ug.