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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ6702 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- DNA Ligase 3 Antibody / LIG3
- Antibody type
- Polyclonal
- Description
- This highly specific DNA Ligase 3 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the DNA Ligase 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) human HEK293, 3) human PC-3, 4) human ThP-1, 5) rat PC-12 and 6) mouse RAW264.7 cell lysate with DNA Ligase 3 antibody. Predicted molecular weight ~113 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human K562 cells with DNA Ligase 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNA Ligase III antibody.
