Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-30948 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TOE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Distinct Poly(A) nucleases have differential impact on sut-2 dependent tauopathy phenotypes.
Kow RL, Strovas TJ, McMillan PJ, Jacobi AM, Behlke MA, Saxton AD, Latimer CS, Keene CD, Kraemer BC
Neurobiology of disease 2021 Jan;147:105148
Neurobiology of disease 2021 Jan;147:105148
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TOE1 in paraformaldehyde-fixed HeLa cells using a TOE1 polyclonal antibody (Product # PA5-30948) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7. TOE1 expression levels parallel MSUT2 expression levels in Alzheimer's disease brain, suggesting the mechanisms altering MSUT2 expression and tauopathy may also affect TOE1 expression. Representative brain sections of postmortem brain frontal cortex from a neurologically normal control and an MSUT2 positive or MSUT2 depleted AD case stained with MSUT2 (a) or TOE1 (b). AD cases that were positive for MSUT2 had robust levels of TOE1, while AD cases depleted of MSUT2 were also deficient in TOE1 immunoreactivity. Representative sections of postmortem cerebral cortex from TOE1 positive or TOE1 depleted AD cases stained with AT180 (detects phospho Thr231 of tau protein) (c) or NeuN (e). The AD brains with depleted levels of mSUT2 (a) and TOE1 (b) demonstrated increased pTau burden (c,d) and increased neuronal loss as indicated by a decrease in NeuN immunoreactivity (e,f). (d,f) Densitometry analysis of AT180 and NeuN positive reactivity in TOE1 positive AD cases ( n = 8) compared to TOE1 depleted AD cases ( n = 7). (AT180, * p = 0.015 and NeuN, ** p = 0.0056 by two-tailed Student's t-test). Scale bars = 100 mum.
