Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B404 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B404, RRID:AB_2299919
- Product name
- IHC-plus™ PNKP Antibody LS-B404
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -20°C.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-PNK Antibody - Western Blot. Western blot of Affinity Purified anti-PNK antibody shows detection of a 57 kD band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature. Signal was detected using standard techniques.