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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB11245 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11245, RRID:AB_1711432
- Product name
- PNKP polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PNKP.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Spectroscopic studies of DNA and ATP binding to human polynucleotide kinase: evidence for a ternary complex.
Involvement of human polynucleotide kinase in double-strand break repair by non-homologous end joining.
Molecular characterization of a human DNA kinase.
Mani RS, Karimi-Busheri F, Fanta M, Cass CE, Weinfeld M
Biochemistry 2003 Oct 21;42(41):12077-84
Biochemistry 2003 Oct 21;42(41):12077-84
Involvement of human polynucleotide kinase in double-strand break repair by non-homologous end joining.
Chappell C, Hanakahi LA, Karimi-Busheri F, Weinfeld M, West SC
The EMBO journal 2002 Jun 3;21(11):2827-32
The EMBO journal 2002 Jun 3;21(11):2827-32
Molecular characterization of a human DNA kinase.
Karimi-Busheri F, Daly G, Robins P, Canas B, Pappin DJ, Sgouros J, Miller GG, Fakhrai H, Davis EM, Le Beau MM, Weinfeld M
The Journal of biological chemistry 1999 Aug 20;274(34):24187-94
The Journal of biological chemistry 1999 Aug 20;274(34):24187-94
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using PNKP polyclonal antibody (Cat # PAB11245) shows detection of a 57 kDa band corresponding to :Lane 1, human PNKP in a Y190 yeast cell lysate.Lane 2, Y190 yeast cell lysate + human PNKP (Gal DNA BP).Lane 3, EM9 XH Chinese hamster ovary cell lysate.Lane 4, EM9 XH Chinese hamster ovary cell lysate + human PNK.Lane 5, a HeLa cell lysate.Approximately 10 ug of lysate was run on SDS-PAGE and reaction with a 1:1000 dilution of PNKP polyclonal antibody.
