PA5-93150

antibody from Invitrogen Antibodies

Targeting: PNKP PNK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-93150

Provider

Invitrogen Antibodies

Product name

PNK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RTVAVKQLGV NPSTTGTQEL KPGLEGSLGV GDTLYLVNGL HPLTLRWEET RTPESQPDTP PGTPLVSQDE KRDAELPKKR MRKSNPGWEN LEKLLVFTAA GVKPQGKVAG FDLDGTLITT RSGKVFPTGP SDWRILYPEI PRKLRELEAE GYKLVIFTNQ MSIGRGKLPA EEFKAKVEAV VEKLGVPFQV LVATHAGLYR KPVTGMWDHL QEQANDGTPI SIGDSIFVGD AAGRPANWAP GRKKKDFSCA DRLFALNLGL P; Positive Samples: HL-60, HepG2, Rat liver; Cellular Location: Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.68 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using PNKP Polyclonal antibody (Product # PA5-93150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PNK in extracts of various cell lines using PNK Polyclonal Antibody (Product # PA5-93150) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PNK was performed in GFP-RNF168 transgenic U2OS cells using PNK Polyclonal Antibody (Product # PA5-93150).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PNK in paraffin-embedded rat kidney using PNK Polyclonal Antibody (Product # PA5-93150) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PNK in paraffin-embedded human lung cancer using PNK Polyclonal Antibody (Product # PA5-93150) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PNK in paraffin-embedded human prostate cancer using PNK Polyclonal Antibody (Product # PA5-93150) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PNK in paraffin-embedded mouse liver using PNK Polyclonal Antibody (Product # PA5-93150) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PNK in paraffin-embedded mouse heart using PNK Polyclonal Antibody (Product # PA5-93150) at a dilution of 1:100.