Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- PA5-93150 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PNK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RTVAVKQLGV NPSTTGTQEL KPGLEGSLGV GDTLYLVNGL HPLTLRWEET RTPESQPDTP PGTPLVSQDE KRDAELPKKR MRKSNPGWEN LEKLLVFTAA GVKPQGKVAG FDLDGTLITT RSGKVFPTGP SDWRILYPEI PRKLRELEAE GYKLVIFTNQ MSIGRGKLPA EEFKAKVEAV VEKLGVPFQV LVATHAGLYR KPVTGMWDHL QEQANDGTPI SIGDSIFVGD AAGRPANWAP GRKKKDFSCA DRLFALNLGL P; Positive Samples: HL-60, HepG2, Rat liver; Cellular Location: Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.68 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PNK was performed in GFP-RNF168 transgenic U2OS cells using PNK Polyclonal Antibody (Product # PA5-93150).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PNK in GFP-RNF168 transgenic U2OS cells. Samples were incubated with PNK Polyclonal antibody (Product # PA5-93150). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PNK in paraffin-embedded human lung cancer. Samples were incubated with PNK Polyclonal antibody (Product # PA5-93150) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PNK in paraffin-embedded mouse heart. Samples were incubated with PNK Polyclonal antibody (Product # PA5-93150) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PNK in paraffin-embedded rat kidney. Samples were incubated with PNK Polyclonal antibody (Product # PA5-93150) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.