PA5-93150

antibody from Invitrogen Antibodies

Targeting: PNKP PNK

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-93150

Provider

Invitrogen Antibodies

Product name

PNK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RTVAVKQLGV NPSTTGTQEL KPGLEGSLGV GDTLYLVNGL HPLTLRWEET RTPESQPDTP PGTPLVSQDE KRDAELPKKR MRKSNPGWEN LEKLLVFTAA GVKPQGKVAG FDLDGTLITT RSGKVFPTGP SDWRILYPEI PRKLRELEAE GYKLVIFTNQ MSIGRGKLPA EEFKAKVEAV VEKLGVPFQV LVATHAGLYR KPVTGMWDHL QEQANDGTPI SIGDSIFVGD AAGRPANWAP GRKKKDFSCA DRLFALNLGL P; Positive Samples: HL-60, HepG2, Rat liver; Cellular Location: Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.68 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PNK was performed in GFP-RNF168 transgenic U2OS cells using PNK Polyclonal Antibody (Product # PA5-93150).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PNK in GFP-RNF168 transgenic U2OS cells. Samples were incubated with PNK Polyclonal antibody (Product # PA5-93150). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PNK in paraffin-embedded human lung cancer. Samples were incubated with PNK Polyclonal antibody (Product # PA5-93150) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PNK in paraffin-embedded mouse heart. Samples were incubated with PNK Polyclonal antibody (Product # PA5-93150) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PNK in paraffin-embedded rat kidney. Samples were incubated with PNK Polyclonal antibody (Product # PA5-93150) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.