Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-87257 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87257, RRID:AB_11034135
- Product name
- Rabbit Polyclonal PNK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PNK antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Mechanism for recycling tRNAs on stalled ribosomes.
MAPK Pathway Suppression Unmasks Latent DNA Repair Defects and Confers a Chemical Synthetic Vulnerability in BRAF-, NRAS-, and NF1-Mutant Melanomas.
Mutant huntingtin impairs PNKP and ATXN3, disrupting DNA repair and transcription.
In vivo phosphoproteomics reveals kinase activity profiles that predict treatment outcome in triple-negative breast cancer.
Polynucleotide kinase-phosphatase enables neurogenesis via multiple DNA repair pathways to maintain genome stability.
Yip MCJ, Keszei AFA, Feng Q, Chu V, McKenna MJ, Shao S
Nature structural & molecular biology 2019 May;26(5):343-349
Nature structural & molecular biology 2019 May;26(5):343-349
MAPK Pathway Suppression Unmasks Latent DNA Repair Defects and Confers a Chemical Synthetic Vulnerability in BRAF-, NRAS-, and NF1-Mutant Melanomas.
Maertens O, Kuzmickas R, Manchester HE, Emerson CE, Gavin AG, Guild CJ, Wong TC, De Raedt T, Bowman-Colin C, Hatchi E, Garraway LA, Flaherty KT, Pathania S, Elledge SJ, Cichowski K
Cancer discovery 2019 Apr;9(4):526-545
Cancer discovery 2019 Apr;9(4):526-545
Mutant huntingtin impairs PNKP and ATXN3, disrupting DNA repair and transcription.
Gao R, Chakraborty A, Geater C, Pradhan S, Gordon KL, Snowden J, Yuan S, Dickey AS, Choudhary S, Ashizawa T, Ellerby LM, La Spada AR, Thompson LM, Hazra TK, Sarkar PS
eLife 2019 Apr 17;8
eLife 2019 Apr 17;8
In vivo phosphoproteomics reveals kinase activity profiles that predict treatment outcome in triple-negative breast cancer.
Zagorac I, Fernandez-Gaitero S, Penning R, Post H, Bueno MJ, Mouron S, Manso L, Morente MM, Alonso S, Serra V, Muñoz J, Gómez-López G, Lopez-Acosta JF, Jimenez-Renard V, Gris-Oliver A, Al-Shahrour F, Piñeiro-Yañez E, Montoya-Suarez JL, Apala JV, Moreno-Torres A, Colomer R, Dopazo A, Heck AJR, Altelaar M, Quintela-Fandino M
Nature communications 2018 Aug 29;9(1):3501
Nature communications 2018 Aug 29;9(1):3501
Polynucleotide kinase-phosphatase enables neurogenesis via multiple DNA repair pathways to maintain genome stability.
Shimada M, Dumitrache LC, Russell HR, McKinnon PJ
The EMBO journal 2015 Oct 1;34(19):2465-80
The EMBO journal 2015 Oct 1;34(19):2465-80
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PNK Antibody [NBP1-87257] - Simple Western lane view shows a specific band for PNK in 0.5 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PNK Antibody [NBP1-87257] - Electropherogram image(s) of corresponding Simple Western lane view. PNK antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PNK Antibody [NBP1-87257] - Analysis in human cell line HEK 293.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PNK Antibody [NBP1-87257] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PNKP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PNK Antibody [NBP1-87257] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.