MA1-25038

antibody from Invitrogen Antibodies

Targeting: CGA FSHA, GPA1, GPHa, GPHA1, HCG, LHA, TSHA

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA1-25038

Provider

Invitrogen Antibodies

Product name

CGA Monoclonal Antibody (INN-hFSH-132)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-25038 will not react with glycoprotein hormones of various animal species.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

INN-hFSH-132

Vial size

250 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

A high-throughput analysis of the IDH1(R132H) protein expression in pituitary adenomas.

Casar-Borota O, Øystese KA, Sundström M, Melchior L, Popovic V
Pituitary 2016 Aug;19(4):407-14

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CGA was performed using 70% confluent log phase BeWo cells treated with Protein Transport Inhibitor (1X, 4hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CGA Monoclonal Antibody (INN-hFSH-132) (Product # MA1-25038) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1:3000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents merged image for untreated BeWo cells showing no staining for CGA. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.