LS-C72739

antibody from LSBio

Targeting: KMT2A ALL-1, CXXC7, HRX, HTRX1, MLL, MLL1A, TRX1

Western blot (Enhanced validation)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C72739

Provider

LSBio

Product name

KMT2A / MLL Antibody (N-Terminus) LS-C72739

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse

Host

Rabbit

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WB Analysis: Samples: Nuclear extract (50 ug for WT and KO; 10 ug for EF, EN and EC) from 293T cells that were transfected with MLL1 C-terminal 180 kD fragment (EC), 293T cells transfected with N-terminally truncated MLL1 fragment (EN; N300-tr), 293T cells transfected with MLL1 N-terminal 300 kD fragment (EF), MLL1 +/+ mouse embryonic stem cells (WT), and MLL1 -/- embryonic stem cells (KO). Antibody used at the indicated concentrations. Detection: One second exposure with chemiluminescence.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WB Analysis: Samples: Nuclear extract (50 ug for WT and KO; 10 ug for EF, EN and EC) from 293T cells that were transfected with MLL1 C-terminal 180 kD fragment (EC), 293T cells transfected with N-terminally truncated MLL1 fragment (EN; N300-tr), 293T cells transfected with MLL1 N-terminal 300 kD fragment (EF), MLL1 +/+ mouse embryonic stem cells (WT), and MLL1 -/- embryonic stem cells (KO). Antibody used at the indicated concentrations. Detection: One second exposure with chemiluminescence.