- HPA006717 - Atlas Antibodies XRCC1 antibody

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Enhanced validation

Submitted by: klas2
Enhanced method: Genetic validation
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Experimental details: Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting XRCC1 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting XRCC1 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
Sample type: U-2 OS
Primary Ab dilution: 1:167
Conjugate: Horseradish Peroxidase
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:3000
Knockdown/Genetic Approaches Application: Western blot
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no: s14940
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod no 2: s14941
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Page 3: KD Reagent Page 3
KD Reagent Prod no 3: s14942
Antibody Lot Number: R07487
Appendix: 565c9cfc5ea61.pdf

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-XRCC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-XRCC1 antibody. Remaining relative intensity is presented

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Recombinant expression validation
Main image
Experimental details: Western blot analysis in control (vector only transfected HEK293T lysate) and XRCC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401897).

Enhanced validation

Submitted by: 55af80e3e0991
Enhanced method: Genetic validation
Main image
Experimental details: Confocal images of immunofluorescently stained human U-2 OS cells.The protein XRCC1 is shown in green and the microtubules in red. The image to the left show cells transfected with control siRNA and the image to the right show cells where XRCC1 has been downregulated with specific siRNA.
Sample type: U-2 OS cells
Primary Ab dilution: 1:75
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:800
Knockdown/Genetic Approaches Application: Immunocytochemistry
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod no: s14940
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no 2: s14941
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Prod no 3: s14942
KD Reagent Page 3: KD Reagent Page 3
Antibody Lot Number: R07487
Appendix: 55b0a8f283144.pdf

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human pancreas shows moderate to strong nuclear positivity in exocrine glandular cells.
Sample type: HUMAN

HPA006717