A301-304A

antibody from Invitrogen Antibodies

Targeting: POLI RAD30B, RAD3OB

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-304A

Provider

Invitrogen Antibodies

Product name

Pol Iota Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pol Iota by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Pol Iota antibody (Product # A301-304A; lot A301-304A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pol Iota by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Pol Iota antibody A301-304A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Pol Iota was also immunoprecipitated by rabbit anti-Pol Iota antibody A301-303A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pol Iota by western blot of immunoprecipitaes. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pol Iota antibody (Product # A301-304A; lot A301-304A-1) used for IP at 3 µg/mg lysate. Pol Iota was also immunoprecipitated by rabbit anti-Pol Iota antibody (Product # A301-303A), which recognizes a downstream epitope. For blotting immunoprecipitated Pol Iota, Product # A301-304A was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.