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- Antibody Data
- Antigen structure
- References [28]
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- Product number
- NB100-109 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-109, RRID:AB_10000540
- Product name
- Rabbit Polyclonal MTH1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 ml
- Concentration
- 1.0 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references TH1579, MTH1 inhibitor, delays tumour growth and inhibits metastases development in osteosarcoma model.
The Epstein-Barr virus nuclear antigen-1 upregulates the cellular antioxidant defense to enable B-cell growth transformation and immortalization.
A profile of 8-oxo-dGTPase activities in the NCI-60 human cancer panel: Meta-analytic insight into the regulation and role of MTH1 (NUDT1) gene expression in carcinogenesis.
MTH1 Inhibitor TH588 Disturbs Mitotic Progression and Induces Mitosis-Dependent Accumulation of Genomic 8-oxodG.
Increased MTH1-specific 8-oxodGTPase activity is a hallmark of cancer in colon, lung and pancreatic tissue.
Potent and specific MTH1 inhibitors targeting gastric cancer.
The presumed MTH1-inhibitor TH588 sensitizes colorectal carcinoma cells to ionizing radiation in hypoxia.
Small molecule inducers of ABCA1 and apoE that act through indirect activation of the LXR pathway.
Validating the concept of mutational signatures with isogenic cell models.
The roles of human MTH1, MTH2 and MTH3 proteins in maintaining genome stability under oxidative stress.
Targeted NUDT5 inhibitors block hormone signaling in breast cancer cells.
Human MutT homologue 1 mRNA overexpression correlates to poor response of multiple myeloma.
hMYH and hMTH1 cooperate for survival in mismatch repair defective T-cell acute lymphoblastic leukemia.
Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling.
MutT Homolog 1 (MTH1) maintains multiple KRAS-driven pro-malignant pathways.
MTH1 expression is required for effective transformation by oncogenic HRAS.
Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition.
Crystal structure, biochemical and cellular activities demonstrate separate functions of MTH1 and MTH2.
Stereospecific targeting of MTH1 by (S)-crizotinib as an anticancer strategy.
Acute responses of DNA repair proteins and StarD6 in rat hippocampus after domoic acid-induced excitotoxicity.
8-Oxoguanine causes neurodegeneration during MUTYH-mediated DNA base excision repair.
α-Galactosidase/sucrose kinase (AgaSK), a novel bifunctional enzyme from the human microbiome coupling galactosidase and kinase activities.
Enhanced elimination of oxidized guanine nucleotides inhibits oncogenic RAS-induced DNA damage and premature senescence.
Continuous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence.
Mechanistic approach of contrasting modifying effects of caffeine on carcinogenesis in the rat colon and mammary gland induced with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine.
Mechanistic approach of contrasting modifying effects of caffeine on carcinogenesis in the rat colon and mammary gland induced with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine.
Expression of human MutT homologue (hMTH1) protein in primary non-small-cell lung carcinomas and histologically normal surrounding tissue.
Expression of human MutT homologue (hMTH1) protein in primary non-small-cell lung carcinomas and histologically normal surrounding tissue.
Moukengue B, Brown HK, Charrier C, Battaglia S, Baud'huin M, Quillard T, Pham TM, Pateras IS, Gorgoulis VG, Helleday T, Heymann D, Berglund UW, Ory B, Lamoureux F
EBioMedicine 2020 Mar;53:102704
EBioMedicine 2020 Mar;53:102704
The Epstein-Barr virus nuclear antigen-1 upregulates the cellular antioxidant defense to enable B-cell growth transformation and immortalization.
Wang J, Nagy N, Masucci MG
Oncogene 2020 Jan;39(3):603-616
Oncogene 2020 Jan;39(3):603-616
A profile of 8-oxo-dGTPase activities in the NCI-60 human cancer panel: Meta-analytic insight into the regulation and role of MTH1 (NUDT1) gene expression in carcinogenesis.
Bialkowski K, Kasprzak KS
Free radical biology & medicine 2020 Feb 20;148:1-21
Free radical biology & medicine 2020 Feb 20;148:1-21
MTH1 Inhibitor TH588 Disturbs Mitotic Progression and Induces Mitosis-Dependent Accumulation of Genomic 8-oxodG.
Rudd SG, Gad H, Sanjiv K, Amaral N, Hagenkort A, Groth P, Ström CE, Mortusewicz O, Berglund UW, Helleday T
Cancer research 2020 Apr 20;
Cancer research 2020 Apr 20;
Increased MTH1-specific 8-oxodGTPase activity is a hallmark of cancer in colon, lung and pancreatic tissue.
McPherson LA, Troccoli CI, Ji D, Bowles AE, Gardiner ML, Mohsen MG, Nagathihalli NS, Nguyen DM, Robbins DJ, Merchant NB, Kool ET, Rai P, Ford JM
DNA repair 2019 Nov;83:102644
DNA repair 2019 Nov;83:102644
Potent and specific MTH1 inhibitors targeting gastric cancer.
Zhou W, Ma L, Yang J, Qiao H, Li L, Guo Q, Ma J, Zhao L, Wang J, Jiang G, Wan X, Adam Goscinski M, Ding L, Zheng Y, Li W, Liu H, Suo Z, Zhao W
Cell death & disease 2019 Jun 4;10(6):434
Cell death & disease 2019 Jun 4;10(6):434
The presumed MTH1-inhibitor TH588 sensitizes colorectal carcinoma cells to ionizing radiation in hypoxia.
Pompsch M, Vogel J, Classen F, Kranz P, Iliakis G, Riffkin H, Brockmeier U, Metzen E
BMC cancer 2018 Nov 29;18(1):1190
BMC cancer 2018 Nov 29;18(1):1190
Small molecule inducers of ABCA1 and apoE that act through indirect activation of the LXR pathway.
Fan J, Zhao RQ, Parro C, Zhao W, Chou HY, Robert J, Deeb TZ, Raynoschek C, Barichievy S, Engkvist O, Maresca M, Hicks R, Meuller J, Moss SJ, Brandon NJ, Wood MW, Kulic I, Wellington CL
Journal of lipid research 2018 May;59(5):830-842
Journal of lipid research 2018 May;59(5):830-842
Validating the concept of mutational signatures with isogenic cell models.
Zou X, Owusu M, Harris R, Jackson SP, Loizou JI, Nik-Zainal S
Nature communications 2018 May 1;9(1):1744
Nature communications 2018 May 1;9(1):1744
The roles of human MTH1, MTH2 and MTH3 proteins in maintaining genome stability under oxidative stress.
Hashiguchi K, Hayashi M, Sekiguchi M, Umezu K
Mutation research 2018 Mar;808:10-19
Mutation research 2018 Mar;808:10-19
Targeted NUDT5 inhibitors block hormone signaling in breast cancer cells.
Page BDG, Valerie NCK, Wright RHG, Wallner O, Isaksson R, Carter M, Rudd SG, Loseva O, Jemth AS, Almlöf I, Font-Mateu J, Llona-Minguez S, Baranczewski P, Jeppsson F, Homan E, Almqvist H, Axelsson H, Regmi S, Gustavsson AL, Lundbäck T, Scobie M, Strömberg K, Stenmark P, Beato M, Helleday T
Nature communications 2018 Jan 17;9(1):250
Nature communications 2018 Jan 17;9(1):250
Human MutT homologue 1 mRNA overexpression correlates to poor response of multiple myeloma.
Zhou H, Jian Y, Leng Y, Liu N, Tian Y, Wang G, Gao W, Yang G, Chen W
International journal of hematology 2017 Mar;105(3):318-325
International journal of hematology 2017 Mar;105(3):318-325
hMYH and hMTH1 cooperate for survival in mismatch repair defective T-cell acute lymphoblastic leukemia.
Eshtad S, Mavajian Z, Rudd SG, Visnes T, Boström J, Altun M, Helleday T
Oncogenesis 2016 Dec 5;5(12):e275
Oncogenesis 2016 Dec 5;5(12):e275
Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling.
Huber KV, Olek KM, Müller AC, Tan CS, Bennett KL, Colinge J, Superti-Furga G
Nature methods 2015 Nov;12(11):1055-7
Nature methods 2015 Nov;12(11):1055-7
MutT Homolog 1 (MTH1) maintains multiple KRAS-driven pro-malignant pathways.
Patel A, Burton DG, Halvorsen K, Balkan W, Reiner T, Perez-Stable C, Cohen A, Munoz A, Giribaldi MG, Singh S, Robbins DJ, Nguyen DM, Rai P
Oncogene 2015 May 14;34(20):2586-96
Oncogene 2015 May 14;34(20):2586-96
MTH1 expression is required for effective transformation by oncogenic HRAS.
Giribaldi MG, Munoz A, Halvorsen K, Patel A, Rai P
Oncotarget 2015 May 10;6(13):11519-29
Oncotarget 2015 May 10;6(13):11519-29
Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition.
Bonora M, Wieckowsk MR, Chinopoulos C, Kepp O, Kroemer G, Galluzzi L, Pinton P
Oncogene 2015 Mar 19;34(12):1608
Oncogene 2015 Mar 19;34(12):1608
Crystal structure, biochemical and cellular activities demonstrate separate functions of MTH1 and MTH2.
Carter M, Jemth AS, Hagenkort A, Page BD, Gustafsson R, Griese JJ, Gad H, Valerie NC, Desroses M, Boström J, Warpman Berglund U, Helleday T, Stenmark P
Nature communications 2015 Aug 4;6:7871
Nature communications 2015 Aug 4;6:7871
Stereospecific targeting of MTH1 by (S)-crizotinib as an anticancer strategy.
Huber KV, Salah E, Radic B, Gridling M, Elkins JM, Stukalov A, Jemth AS, Göktürk C, Sanjiv K, Strömberg K, Pham T, Berglund UW, Colinge J, Bennett KL, Loizou JI, Helleday T, Knapp S, Superti-Furga G
Nature 2014 Apr 10;508(7495):222-7
Nature 2014 Apr 10;508(7495):222-7
Acute responses of DNA repair proteins and StarD6 in rat hippocampus after domoic acid-induced excitotoxicity.
Chang IY, Kim JH, Cho KW, Yoon SP
Acta histochemica 2013 Apr;115(3):234-9
Acta histochemica 2013 Apr;115(3):234-9
8-Oxoguanine causes neurodegeneration during MUTYH-mediated DNA base excision repair.
Sheng Z, Oka S, Tsuchimoto D, Abolhassani N, Nomaru H, Sakumi K, Yamada H, Nakabeppu Y
The Journal of clinical investigation 2012 Dec;122(12):4344-61
The Journal of clinical investigation 2012 Dec;122(12):4344-61
α-Galactosidase/sucrose kinase (AgaSK), a novel bifunctional enzyme from the human microbiome coupling galactosidase and kinase activities.
Bruel L, Sulzenbacher G, Cervera Tison M, Pujol A, Nicoletti C, Perrier J, Galinier A, Ropartz D, Fons M, Pompeo F, Giardina T
The Journal of biological chemistry 2011 Nov 25;286(47):40814-23
The Journal of biological chemistry 2011 Nov 25;286(47):40814-23
Enhanced elimination of oxidized guanine nucleotides inhibits oncogenic RAS-induced DNA damage and premature senescence.
Rai P, Young JJ, Burton DG, Giribaldi MG, Onder TT, Weinberg RA
Oncogene 2011 Mar 24;30(12):1489-96
Oncogene 2011 Mar 24;30(12):1489-96
Continuous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence.
Rai P, Onder TT, Young JJ, McFaline JL, Pang B, Dedon PC, Weinberg RA
Proceedings of the National Academy of Sciences of the United States of America 2009 Jan 6;106(1):169-74
Proceedings of the National Academy of Sciences of the United States of America 2009 Jan 6;106(1):169-74
Mechanistic approach of contrasting modifying effects of caffeine on carcinogenesis in the rat colon and mammary gland induced with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine.
Takeshita F, Ogawa K, Asamoto M, Shirai T
Cancer letters 2003 May 8;194(1):25-35
Cancer letters 2003 May 8;194(1):25-35
Mechanistic approach of contrasting modifying effects of caffeine on carcinogenesis in the rat colon and mammary gland induced with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine.
Takeshita F, Ogawa K, Asamoto M, Shirai T
Cancer letters 2003 May 8;194(1):25-35
Cancer letters 2003 May 8;194(1):25-35
Expression of human MutT homologue (hMTH1) protein in primary non-small-cell lung carcinomas and histologically normal surrounding tissue.
Kennedy CH, Pass HI, Mitchell JB
Free radical biology & medicine 2003 Jun 1;34(11):1447-57
Free radical biology & medicine 2003 Jun 1;34(11):1447-57
Expression of human MutT homologue (hMTH1) protein in primary non-small-cell lung carcinomas and histologically normal surrounding tissue.
Kennedy CH, Pass HI, Mitchell JB
Free radical biology & medicine 2003 Jun 1;34(11):1447-57
Free radical biology & medicine 2003 Jun 1;34(11):1447-57
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: MTH1 Antibody [NB100-109] - Simple Western lane view shows a specific band for MTH1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTH1 Antibody [NB100-109] - Analysis of MTH1 in 1. Ntera2, 2. MCF7 cell lysate, 3. A431 cell lysate and 4. COS7 cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTH1 Antibody [NB100-109] - MTH1 antibody was tested in the labeled lanes.
