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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30475 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ORC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.52 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ORC3 Polyclonal Antibody (Product # PA5-30475). Sample (30 µg of whole cell lysate). Lane A: H1299. 7.5% SDS PAGE. ORC3 Polyclonal Antibody (Product # PA5-30475) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ORC3 Polyclonal Antibody detects ORC3L protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORC3L protein stained by ORC3 Polyclonal Antibody (Product # PA5-30475) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using ORC3L (Product # PA5-30475) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
