Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- PAB2168 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2168, RRID:AB_1579183
- Product name
- PRMT1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRMT1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Genomic organization, physical mapping, and expression analysis of the human protein arginine methyltransferase 1 gene.
Crystal structure of the conserved core of protein arginine methyltransferase PRMT3.
Identification and characterization of two putative human arginine methyltransferases (HRMT1L1 and HRMT1L2).
Scorilas A, Black MH, Talieri M, Diamandis EP
Biochemical and biophysical research communications 2000 Nov 19;278(2):349-59
Biochemical and biophysical research communications 2000 Nov 19;278(2):349-59
Crystal structure of the conserved core of protein arginine methyltransferase PRMT3.
Zhang X, Zhou L, Cheng X
The EMBO journal 2000 Jul 17;19(14):3509-19
The EMBO journal 2000 Jul 17;19(14):3509-19
Identification and characterization of two putative human arginine methyltransferases (HRMT1L1 and HRMT1L2).
Scott HS, Antonarakis SE, Lalioti MD, Rossier C, Silver PA, Henry MF
Genomics 1998 Mar 15;48(3):330-40
Genomics 1998 Mar 15;48(3):330-40
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRMT1 (arrow) using rabbit PRMT1 polyclonal antibody (Cat # PAB2168). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)