Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX108749 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX108749, RRID:AB_1951746
- Product name
- RPA70 antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse liver 7.5% SDS PAGE GTX108749 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ug whole cell lysate)A:H1299B:HeLa S3(GTX14654)7.5% SDS PAGEGTX108749 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RPA70 antibody detects RPA70 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 antibody (GTX108749) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit (GTX108749) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of RPA70 protein from 293T whole cell extracts using 5 £gg of RPA70 antibody [C1C3] (GTX108749).Western blot analysis was performed using RPA70 antibody [C1C3] (GTX108749).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit (GTX108749) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RPA70 antibody [C1C3] detects RPA1 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. RPA70 antibody [C1C3] (GTX108749) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RPA70 antibody [C1C3] detects RPA70 protein at cytoplasm and nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung papillary adenocarcinoma. RPA70 antibody [C1C3] (GTX108749) diluted at 1:250.
