Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- ARG54143 - Provider product page
- Provider
- Arigo
- Product name
- anti-RPA70 antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human RPA70 protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8C3-D12-H10
- Vial size
- 100 µl
- Concentration
- 0.8 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: RPA70 in HeLa, A431, MCF7, COS7, HT1080 and K562 cell lysates stained with RPA70 antibody [8C3-D12-H10] (ARG54143) (diluted in 1: 1000). Predicted band size: 70KDa. Observed band size: 70KDa.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: RPA70 in HeLa, 293T, C6, 3T3 and K562 cell lysates stained with RPA70 antibody [8C3-D12-H10] (ARG54143) (diluted in 1: 1000). Predicted band size: 70KDa. Observed band size: 70KDa.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: RPA70 in HUVEC, Hacat, 3T3, MCF7 and A549 cell lysates stained with RPA70 antibody [8C3-D12-H10] (ARG54143) (diluted in 1: 2000). Predicted band size: 70KDa. Observed band size: 70KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells fixed in 1% Paraformaldehyde and stained with RPA70 antibody [8C3-D12-H10] (ARG54143) (diluted in 1:100).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: HeLa cell lysates immunoprecipitated and stained by RPA70 antibody [8C3-D12-H10] (ARG54143).