AM06744PU-N

antibody from OriGene

Targeting: RPA1 HSSB, REPA1, RF-A, RP-A, RPA70

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM06744PU-N

Provider

OriGene

Product name

RPA70 (RPA1) mouse monoclonal antibody, clone 4C4, Purified

Antibody type

Monoclonal

Description

RPA70 (RPA1) mouse monoclonal antibody, clone 4C4, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

RPA1

Isotype

IgG

Antibody clone number

4C4

Vial size

100 µg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Left: Western blot analysis using RPA1 mAb against human RPA1 (AA: 308-513) recombinant protein (Expected MW is 48.3 kDa). Right: Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562(3), A431(4), and COS-7 (6) cell lysate.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using RPA1 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using RPA1 antibody with DAB staining.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow Cytometric analysis of Jurkat cells using RPA1 antibody (green) and negative control (red).

Validation comment

FC