Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- STJ99205 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-RPA1 antibody [8C3-D12-H10] (STJ99205)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Replication Protein A 70 Kda Dna-Binding Subunit is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of RPA70 in HUVEC, Hacat, 3T3, MCF7 and A549 cell lysates using RPA70 mouse mAb (1:2000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of RPA70 in Hela, 293T, C6, 3T3 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of RPA70 in Hela, A431, MCF7, COS7, HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA70 mouse mAb (dilution 1:100).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using RPA70 mouse mAb.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA