M01317-2

antibody from Boster Biological Technology

Targeting: RPA1 HSSB, REPA1, RF-A, RP-A, RPA70

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M01317-2

Provider

Boster Biological Technology

Product name

Anti-RPA70 RPA1 Antibody Picoband™ (monoclonal, 11H4)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for RPA70 detection. Tested with WB, IHC-P, IF, FCM in Human;Mouse;Monkey.

Reactivity

Human, Mouse, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

11H4

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of RPA70 using anti- RPA70 antibody (M01317-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: MCF-7 whole cell lysates, Lane 3: K562 whole cell lysates, Lane 4: COS-7 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: A549 whole cell lysates, Lane 7: HEPG2 whole cell lysates, Lane 8: PC-3 whole cell lysates, Lane 9: HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- RPA70 antigen affinity purified monoclonal antibody (Catalog # M01317-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RPA70 at approximately 12KD. The expected band size for RPA70 is at 12KD.

Additional image