PA5-17377

HSSB, REPA1, RF-A, RP-A, RPA70

Antibody data

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Validation data

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RPA70 in extracts from A431 and K562 cells using RPA70 polyclonal antibody (Product # PA5-17377).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of RPA70 was achieved by transfecting HeLa with RPA70 specific siRNAs (Silencer® select Product # s12127, s12129). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the RPA70 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RPA70 Polyclonal Antibody (Product # PA5-17377, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RPA70.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-RPA70 Polyclonal Antibody(Product # PA5-17377) and a 68kDa band corresponding to RPA70 was observed across cell lines tested. Nuclear enriched extracts (30µg lysate) of HeLa (Lane 1), A-431 (Lane 2) and K-562 (Lane 3) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).