PA5-21976

HSSB, REPA1, RF-A, RP-A, RPA70

Antibody data

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Validation data

Product number: PA5-21976
Provider: Invitrogen Antibodies
Product name: Anti-RPA70 Polyclonal Antibody
Provider product page: Invitrogen Antibodies - PA5-21976
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: PA5-21976 targets RPA 70 kDa subunit in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples. The PA5-21976 immunogen is a recombinant fragment corresponding to a region within amino acids 369 and 616 of RPA70 (Uniprot ID#P27694). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.4 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RPA 70 kDa subunit using Various whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a RPA 70 kDa subunit polyclonal antibody (Product # PA5-21976) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of RPA70 was achieved by transfecting HeLa cells with RPA70 specific siRNAs (Silencer® select Product # s12127, s12129). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the RPA70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with RPA70 Polyclonal Antibody (Product # PA5-21976, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RPA70.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), K-562 (Lane 3), Hep G2 (Lane 4), HCT 116 (Lane 5), Panc-1 (Lane 6) and U-2 OS (Lane 7). The blot was probed with Anti- PCNA Polyclonal Antibody (Product # MA5-17166, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/mL, 1:4000 dilution). A 70 kDa band corresponding to RPA70 was observed across the cell lines tested with additional non specific band at 35 kDa.