Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [7]
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Validation data
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- Product number
- PA5-55400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPACA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: REVILTNGCP GGESKCVVRV EECRGPTDCG WGKPISESLE SVRLACIHTS PLNRFKYMWK L Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 89%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPACA1 in control (vector only transfected HEK293T lysate) and SPACA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SPACA1 Polyclonal Antibody (Product # PA5-55400).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA1 in human kidney using SPACA1 Polyclonal Antibody (Product # PA5-55400).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA1 in human cerebral cortex using SPACA1 Polyclonal Antibody (Product # PA5-55400).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA1 in human liver using SPACA1 Polyclonal Antibody (Product # PA5-55400).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA1 in human testis and endometrium tissues using SPACA1 Polyclonal Antibody (Product # PA5-55400). Corresponding SPACA1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA1 in human cerebral cortex, kidney, liver and testis using SPACA1 Polyclonal Antibody (Product # PA5-55400) (A) shows similar protein distribution across tissues to an independent SPACA1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA1 in human endometrium using SPACA1 Polyclonal Antibody (Product # PA5-55400) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPACA1 in human testis using SPACA1 Polyclonal Antibody (Product # PA5-55400) shows high expression.