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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- A304-502A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AE2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human AE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-AE2 antibody A304-502A (lot A304-502A-1) used for IP at 6 µg per reaction. AE2 was also immunoprecipitated by rabbit anti-AE2 antibody A304-503A. For blotting immunoprecipitated AE2, A304-503A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human AE2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-AE2 antibody A304-502A (lot A304-502A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human AE2 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- AE2 (Cat. No. A304-502A Lot1, left image and Cat. No. A304-503A Lot1, right image) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
