Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-19993 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CARD9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- In Western blot applications, this antibody detects a band at ~59kDa. A suggested positive control is PC-3 cell lysate.
- Concentration
- 1 mg/mL
Submitted references Structures of autoinhibited and polymerized forms of CARD9 reveal mechanisms of CARD9 and CARD11 activation.
Holliday MJ, Witt A, RodrÃguez Gama A, Walters BT, Arthur CP, Halfmann R, Rohou A, Dueber EC, Fairbrother WJ
Nature communications 2019 Jul 11;10(1):3070
Nature communications 2019 Jul 11;10(1):3070
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MDA-MB-361 (A) and PC-3 (B) cell lysate using a CARD9 polyclonal antibody (Product # PA5-19993) at 2.5 µg /mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 Polyclonal Antibody (Product # PA5-19993) at 2.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of CARD9 in K562 cells with CARD9 Polyclonal Antibody (Product # PA5-19993) at 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of K562 cells using a CARD9 polyclonal antibody (Product # PA5-19993) at a 10 µg/mL dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 The CARD11 CARD-coiled-coil interface is weaker than that of CARD9, but is reinforced by the ID. a Cartoon schematic of the domain architecture of CARD9, CARD11-DeltaID, and CARD11 along with the chimeric constructs CARD9 1-131 /CARD11-DeltaID and CARD11 1-143 /CARD9, wherein the N-terminal residues comprising the CARD, linker, and CARD-interacting stretch of the coil-coil are swapped between the two proteins (See Supplementary Fig. 1B ). b Normalized NF-kappaB activation in HEK-293 cells upon overexpression of the indicated constructs. Three biologically independent experiments were undertaken, the outcomes of which are shown as dots. The mean value for each construct is depicted as a gray bar. Dotted line represents the value of the CARD9 WT construct, to which the other values were normalized. Representative western blot is shown for CARD9 and CARD11 constructs, using antibodies specific for regions outside of the N-terminal swapped domain or ID. Source data are provided in Source Data file