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antibody from Novus Biologicals
Targeting: GFRA1
GDNFR, GDNFRA, GFR-ALPHA-1, RET1L, RETL1, TRNR1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF560 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal GFR alpha-1/GDNF R alpha-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat GFR alpha-1/GDNF R alpha-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human GFR alpha-1 is observed and less than 1% cross-reactivity with recombinant mouse GFR alpha-2 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references PLZF(pos)c-KIT(pos)-delineated A(1)-A(4)-differentiating spermatogonia by subset and stage detection upon Bouin fixation.
GDNF and GFRα1 Are Required for Proper Integration of Adult-Born Hippocampal Neurons.
The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.
Advanced immunostaining approaches to study early male germ cell development.
Prolyl isomerase Pin1 is required sperm production by promoting mitosis progression of spermatogonial stem cells.
The adult human testis transcriptional cell atlas.
Aging-related limit of exercise efficacy on motor decline.
Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons.
Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.
PAX7 expression defines germline stem cells in the adult testis.
LIN28A marks the spermatogonial progenitor population and regulates its cyclic expansion.
Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
Retinoic acid availability drives the asynchronous initiation of spermatogonial differentiation in the mouse.
The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3.
Glial cell line-derived neurotrophic factor: an intraovarian factor that enhances oocyte developmental competence in vitro.
Astrocyte and muscle-derived secreted factors differentially regulate motoneuron survival.
Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings.
Nerve growth factor, glial cell line-derived neurotrophic factor and neurturin prevent semaphorin 3A-mediated growth cone collapse in adult sensory neurons.
Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo.
Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells.
Tang RL, Fan LQ
Asian journal of andrology 2019 May-Jun;21(3):309-318
Asian journal of andrology 2019 May-Jun;21(3):309-318
GDNF and GFRα1 Are Required for Proper Integration of Adult-Born Hippocampal Neurons.
Bonafina A, Trinchero MF, Ríos AS, Bekinschtein P, Schinder AF, Paratcha G, Ledda F
Cell reports 2019 Dec 24;29(13):4308-4319.e4
Cell reports 2019 Dec 24;29(13):4308-4319.e4
The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.
Hermann BP, Cheng K, Singh A, Roa-De La Cruz L, Mutoji KN, Chen IC, Gildersleeve H, Lehle JD, Mayo M, Westernströer B, Law NC, Oatley MJ, Velte EK, Niedenberger BA, Fritze D, Silber S, Geyer CB, Oatley JM, McCarrey JR
Cell reports 2018 Nov 6;25(6):1650-1667.e8
Cell reports 2018 Nov 6;25(6):1650-1667.e8
Advanced immunostaining approaches to study early male germ cell development.
Niedenberger BA, Geyer CB
Stem cell research 2018 Mar;27:162-168
Stem cell research 2018 Mar;27:162-168
Prolyl isomerase Pin1 is required sperm production by promoting mitosis progression of spermatogonial stem cells.
Kurita-Suzuki A, Kamo Y, Uchida C, Tanemura K, Hara K, Uchida T
Biochemical and biophysical research communications 2018 Feb 26;497(1):388-393
Biochemical and biophysical research communications 2018 Feb 26;497(1):388-393
The adult human testis transcriptional cell atlas.
Guo J, Grow EJ, Mlcochova H, Maher GJ, Lindskog C, Nie X, Guo Y, Takei Y, Yun J, Cai L, Kim R, Carrell DT, Goriely A, Hotaling JM, Cairns BR
Cell research 2018 Dec;28(12):1141-1157
Cell research 2018 Dec;28(12):1141-1157
Aging-related limit of exercise efficacy on motor decline.
Arnold JC, Cantu MA, Kasanga EA, Nejtek VA, Papa EV, Bugnariu N, Salvatore MF
PloS one 2017;12(11):e0188538
PloS one 2017;12(11):e0188538
Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons.
Schaller S, Buttigieg D, Alory A, Jacquier A, Barad M, Merchant M, Gentien D, de la Grange P, Haase G
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 21;114(12):E2486-E2493
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 21;114(12):E2486-E2493
Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.
Bye CR, Jönsson ME, Björklund A, Parish CL, Thompson LH
Proceedings of the National Academy of Sciences of the United States of America 2015 Apr 14;112(15):E1946-55
Proceedings of the National Academy of Sciences of the United States of America 2015 Apr 14;112(15):E1946-55
PAX7 expression defines germline stem cells in the adult testis.
Aloisio GM, Nakada Y, Saatcioglu HD, Peña CG, Baker MD, Tarnawa ED, Mukherjee J, Manjunath H, Bugde A, Sengupta AL, Amatruda JF, Cuevas I, Hamra FK, Castrillon DH
The Journal of clinical investigation 2014 Sep;124(9):3929-44
The Journal of clinical investigation 2014 Sep;124(9):3929-44
LIN28A marks the spermatogonial progenitor population and regulates its cyclic expansion.
Chakraborty P, Buaas FW, Sharma M, Snyder E, de Rooij DG, Braun RE
Stem cells (Dayton, Ohio) 2014 Apr;32(4):860-73
Stem cells (Dayton, Ohio) 2014 Apr;32(4):860-73
Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
Euteneuer S, Yang KH, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan AF
Molecular and cellular neurosciences 2013 May;54:30-43
Molecular and cellular neurosciences 2013 May;54:30-43
Retinoic acid availability drives the asynchronous initiation of spermatogonial differentiation in the mouse.
Snyder EM, Small C, Griswold MD
Biology of reproduction 2010 Nov;83(5):783-90
Biology of reproduction 2010 Nov;83(5):783-90
The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3.
Suzuki H, Sada A, Yoshida S, Saga Y
Developmental biology 2009 Dec 15;336(2):222-31
Developmental biology 2009 Dec 15;336(2):222-31
Glial cell line-derived neurotrophic factor: an intraovarian factor that enhances oocyte developmental competence in vitro.
Linher K, Wu D, Li J
Endocrinology 2007 Sep;148(9):4292-301
Endocrinology 2007 Sep;148(9):4292-301
Astrocyte and muscle-derived secreted factors differentially regulate motoneuron survival.
Taylor AR, Gifondorwa DJ, Newbern JM, Robinson MB, Strupe JL, Prevette D, Oppenheim RW, Milligan CE
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Jan 17;27(3):634-44
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Jan 17;27(3):634-44
Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings.
Igarashi Y, Aita M, Suzuki A, Nandasena T, Kawano Y, Nozawa-Inoue K, Maeda T
Neuroscience letters 2007 Feb 2;412(3):222-6
Neuroscience letters 2007 Feb 2;412(3):222-6
Nerve growth factor, glial cell line-derived neurotrophic factor and neurturin prevent semaphorin 3A-mediated growth cone collapse in adult sensory neurons.
Wanigasekara Y, Keast JR
Neuroscience 2006 Oct 13;142(2):369-79
Neuroscience 2006 Oct 13;142(2):369-79
Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo.
Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Aug 16;26(33):8588-99
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Aug 16;26(33):8588-99
Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells.
Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM Jr, Choi DW, Snider BJ
Experimental neurology 2005 Jan;191(1):65-76
Experimental neurology 2005 Jan;191(1):65-76
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Rat GFR alpha-1/GDNF R alpha-1 by Western Blot. Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat GFR alpha-1/GDNF R alpha-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GFR alpha-1/GDNF R alpha-1 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- GFR alpha-1/GDNF R alpha-1 in Rat Spinal Cord. GFR alpha-1/GDNF R alpha-1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GFR alpha-1/GDNF R alpha-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
