MAB560

antibody from R&D Systems

Targeting: GFRA1 GDNFR, GDNFRA, GFR-ALPHA-1, RET1L, RETL1, TRNR1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB560

Provider

R&D Systems

Product name

Rat GFR alpha-1/GDNF R alpha-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects rat GFR alpha-1/GDNF R alpha-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Ret, recombinant human (rh) GFR alpha-2, rhGFR alpha-3 or rmGFR alpha-2 is observed.

Reactivity

Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q62997


Isotype

IgG

Antibody clone number

81401

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).

Euteneuer S, Yang KH, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan AF
Molecular and cellular neurosciences 2013 May;54:30-43

---

Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.

Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2604-14

---

Expression and function of GDNF family ligands and receptors in the carotid body.

Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM Jr
Experimental neurology 2005 Feb;191 Suppl 1:S68-79

---

Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin.

Carmillo P, Dagø L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Grønborg M, Whitty A
Biochemistry 2005 Feb 22;44(7):2545-54

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Rat GFR alpha-1/GDNF R alpha-1 by Western Blot. Western blot shows lysates of rat spinal cord tissue and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat GFR alpha-1/GDNF R alpha-1 Monoclonal Antibody (Catalog # MAB560) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GFR alpha-1/GDNF R alpha-1 at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions (recommended) and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

GFR alpha-1/GDNF R alpha-1 in Rat Spinal Cord. GFR alpha-1/GDNF R alpha-1 was detected in perfusion fixed frozen sections of rat spinal cord using Rat GFR alpha-1/GDNF R alpha-1 Monoclonal Antibody (Catalog # MAB560) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.