Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- BAF560 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat GFR alpha-1/GDNF R alpha-1 in Western blots.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
Q62997
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references GDNF availability determines enteric neuron number by controlling precursor proliferation.
GDNF availability determines enteric neuron number by controlling precursor proliferation.
TGFbeta induces GDNF responsiveness in neurons by recruitment of GFRalpha1 to the plasma membrane.
Gianino S, Grider JR, Cresswell J, Enomoto H, Heuckeroth RO
Development (Cambridge, England) 2003 May;130(10):2187-98
Development (Cambridge, England) 2003 May;130(10):2187-98
GDNF availability determines enteric neuron number by controlling precursor proliferation.
Gianino S, Grider JR, Cresswell J, Enomoto H, Heuckeroth RO
Development (Cambridge, England) 2003 May;130(10):2187-98
Development (Cambridge, England) 2003 May;130(10):2187-98
TGFbeta induces GDNF responsiveness in neurons by recruitment of GFRalpha1 to the plasma membrane.
Peterziel H, Unsicker K, Krieglstein K
The Journal of cell biology 2002 Oct 14;159(1):157-67
The Journal of cell biology 2002 Oct 14;159(1):157-67
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GDNF and GFR alpha-1 in Rat Dorsal Root Ganglia. GDNF and GFR alpha-1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Goat AntiRat Anti-GFR alpha-1 Biotinylated Antigen Affinity-purified Poly-clonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.