PA5-19873

antibody from Invitrogen Antibodies

Targeting: GFRA1 GDNFR, GDNFRA, GFR-ALPHA-1, RET1L, RETL1, TRNR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19873

Provider

Invitrogen Antibodies

Product name

GFR alpha-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is human brain tissue lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of crude membrane fractions of (B), liver (L), kidney (K), and spleen (S), respectively, using a GFR alpha 1 polyclonal antibody (Product # PA5-19873) at 1:500 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha-1 Polyclonal Antibody (Product # PA5-19873) at 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha-1 Polyclonal Antibody (Product # PA5-19873) at 1 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GFR alpha-1 was performed using SH-SY5Y and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GFR alpha-1 Polyclonal Antibody (Product # PA5-19873) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing cytoplasmic and membrane localization of GFR alpha-1. Panel e represents HeLa cells having no significant expression of GFR alpha 1. Panel f represents control SH-SY5Y cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of GFR alpha 1 in human brain tissue with GFR alpha-1 Polyclonal Antibody (Product # PA5-19873) at 20 µg/mL. Red: GFR alpha 1 Blue: DAPI staining

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of GFR alpha 1 in human brain tissue with GFR alpha-1 Polyclonal Antibody (Product # PA5-19873) at 20 µg/mL. Red: GFR alpha 1 Blue: DAPI staining

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha-1 Polyclonal Antibody (Product # PA5-19873) at 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha-1 Polyclonal Antibody (Product # PA5-19873) at 2.5 µg/mL.