MA1-80734

antibody from Invitrogen Antibodies

Targeting: GZMB CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

Provider product page for MA1-80734

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [63]
Comments [0]
Validations
Immunocytochemistry [1]
Other assay [7]

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Product number: MA1-80734 - Provider product page
Provider: Invitrogen Antibodies
Product name: Granzyme B Monoclonal Antibody (GB11)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. Mouse anti Granzyme B antibody, clone GB11 recognizes the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs).
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: GB11
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GZMB protein structure - MA1-80734 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3. The acquisition of resistance to NK cell-mediated lysis correlates with an effector/target interaction default. (A) Confocal microscopy staining of phosphotyrosine (arrowhead, accumulated at the contact zone) on co-harvested tumor (arrow) and NK cells. (B) Confocal microscopy staining of Granzyme B (arrowhead, relocalized at the contact zone) on co-harvested tumor (arrow) and NK cells. Scale bar, 10 um. (C) Statistical analysis of confocal microscopy evaluation of tumor and NK cell contacts, of the establishment of active immune synapses (by phosphotyrosine staining) and of Granzyme B relocalization toward the contact zone. At least 200 tumor cells in 10 different fields were counted for each three independent batches of T1 and each three independent batches of T1R cells. Data represent mean percentage +- standard deviation. (D) Flow cytometry evaluation of conjugate formation between NK cells and tumor cells. Data represent the means of three experiments for the three independent batches of T1 and the three independent batches of T1R cells +- standard deviation. (E) Flow cytometry evaluation of CD107a externalization on NK cell membrane in response to cocultured cells (T1R) in comparison to reference cells (T1). Data represent the means of the three independent batches of T1 and the three independent batches of T1R cells +- standard deviation. (F) Flow cytometry evaluation of main KIR, DNAM1 and NKG2D ligand expression on cocultured cells (T1R) in comparison to ref

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 CXCR5 + NK cells are transcriptionally distinct from CXCR5 - NK cells during chronic SHIV infection. ( A ) Purified NK cells from lymph nodes (LNs) at week 14 after infection were RNA sequenced, and volcano plots were analyzed for differentially expressed transcripts ( n = 4). ( B ) Normalized enrichment scores for upregulated gene sets in CXCR5 + NK cells are depicted. Dashed line indicates normalized enrichment score cutoff of greater than 135 with FDR of less than 0.2. ( C ) GSEA plots comparing CXCR5 + and CXCR5 - NK cells. ( D ) Global gene expression analysis showing gene expression profile for CXCR5 + and CXCR5 - NK cells. The color intensity for heatmaps represents Z score of differential expression by CXCR5 + versus CXCR5 - NK cells, calculated as described in Methods. ( E ) IHC images showing CD20 (blue), CD3 (green), NKG2A (red), and granzyme B (gray) staining of LN sections of SHIV-infected macaques. Scale bar: 50 mum. Arrow indicates NK cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 CD56 expression at immunological synapse and association with granzyme B transfer from NK-92 into CD56-positive target cells. CD56-expressing (hTERT-HME1) and/or CD56-negative (MCF-7, MDA-MB-231 and/or SKBR3) target cells were labeled (panels A and C) or not (panel B) with CFSE and cocultured with NK-92 cells at 1:1 effector-to-target ratio for 2 hours. Cells were then transferred to poly-L-lysin-coated coverslips, fixed, permeabilized and stained as indicated for CD56 (panels A and B), actin (panel B), granzyme b (panel C) and nuclei (DAPI) (panels A, B and C) before analysis by fluorescent microscopy. Images is panel A are for MCF-7 and MDA-MB-231 (CD56-negative targets) and hTERT-HME1 (CD56-positive target) and correspond to representative fields from two or three independent experiments. Images in panel C are for three different synapses observed after 2 hours in the same coculture of NK-92 and hTERT-HME1. ( D ) Graph presenting the mean rates of synapse formation +- SD obtained from six representative fields for CD56-positive target (hTERT-HME1) and nine representative fields for CD56-negative targets (three fields for each cell line MCF-7, MDA-MB-231 and SKBR3). ***p < 0.001.