OAAB03588
antibody from Aviva Systems Biology
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- OAAB03588 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- GZMB antibody - C-terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of GZMB (arrow) using rabbit polyclonal GZMB Antibody (C-term) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the GZMB gene.
- Sample type
- lysatestransiently transfected with the GZMB gene.
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of GZMB Antibody (C-term) in HL-60 cell lysates (35ug/lane). GZMB (arrow) was detected using the purified Pab.
- Sample type
- HL-60 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- GZMB Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- human Lymph tissue
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- GZMB Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- HL-60 cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol