TA505931

antibody from OriGene

Targeting: GZMB CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505931

Provider

OriGene

Proper citation

OriGene Cat#TA505931, RRID:AB_2623479

Product name

GZMB (Granzyme B) mouse monoclonal antibody, clone OTI4E4 (formerly 4E4)

Antibody type

Monoclonal

Description

GZMB (Granzyme B) mouse monoclonal antibody, clone OTI4E4 (formerly 4E4)

Host

Mouse

Conjugate

Unconjugated

Epitope

GZMB

Isotype

IgG

Antibody clone number

OTI4E4

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GZMB (RC206495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GZMB.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-GZMB monoclonal antibody.(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-GZMB mouse monoclonal antibody (TA505931) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GZMB(RC206495).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GZMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505931)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-GZMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505931)

Validation comment

IHC