AF1865
antibody from R&D Systems
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF1865 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Granzyme B Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Granzyme B in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) rhGranzyme A, rhGranzyme B, recombinant mouse (rm) Granzyme D, rmGranzyme G, and rhGranzyme H is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P04187
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references PARP Inhibitor Efficacy Depends on CD8(+) T-cell Recruitment via Intratumoral STING Pathway Activation in BRCA-Deficient Models of Triple-Negative Breast Cancer.
Novel recombinant DNA vaccine candidates for human respiratory syncytial virus: Preclinical evaluation of immunogenicity and protection efficiency.
Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice.
Endogenous retinoids in the pathogenesis of alopecia areata.
Serine protease inhibitor 6 protects cytotoxic T cells from self-inflicted injury by ensuring the integrity of cytotoxic granules.
Pantelidou C, Sonzogni O, De Oliveria Taveira M, Mehta AK, Kothari A, Wang D, Visal T, Li MK, Pinto J, Castrillon JA, Cheney EM, Bouwman P, Jonkers J, Rottenberg S, Guerriero JL, Wulf GM, Shapiro GI
Cancer discovery 2019 Jun;9(6):722-737
Cancer discovery 2019 Jun;9(6):722-737
Novel recombinant DNA vaccine candidates for human respiratory syncytial virus: Preclinical evaluation of immunogenicity and protection efficiency.
Farrag MA, Amer HM, Öhlschläger P, Hamad ME, Almajhdi FN
Human vaccines & immunotherapeutics 2017 Jul 3;13(7):1586-1597
Human vaccines & immunotherapeutics 2017 Jul 3;13(7):1586-1597
Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice.
Whitehead M, Ohlschläger P, Almajhdi FN, Alloza L, Marzábal P, Meyers AE, Hitzeroth II, Rybicki EP
BMC cancer 2014 May 24;14:367
BMC cancer 2014 May 24;14:367
Endogenous retinoids in the pathogenesis of alopecia areata.
Duncan FJ, Silva KA, Johnson CJ, King BL, Szatkiewicz JP, Kamdar SP, Ong DE, Napoli JL, Wang J, King LE Jr, Whiting DA, McElwee KJ, Sundberg JP, Everts HB
The Journal of investigative dermatology 2013 Feb;133(2):334-43
The Journal of investigative dermatology 2013 Feb;133(2):334-43
Serine protease inhibitor 6 protects cytotoxic T cells from self-inflicted injury by ensuring the integrity of cytotoxic granules.
Zhang M, Park SM, Wang Y, Shah R, Liu N, Murmann AE, Wang CR, Peter ME, Ashton-Rickardt PG
Immunity 2006 Apr;24(4):451-61
Immunity 2006 Apr;24(4):451-61
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Granzyme B in Mouse Splenocytes. Granzyme B was detected in immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.