NBP2-59678
antibody from Novus Biologicals
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-59678 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Granzyme B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Granzyme B Antibody [NBP2-59678] - Total protein from human spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Granzyme B in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP2-59678] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human tonsil (NBP2-30207) using Granzyme B antibody at 1:500 dilution. The primary antibody bound to antigens was detected using HRP conjugated secondary antibody which followed staining with DAB and counterstained with hematoxylin. The antibody generated a specific punctate staining pattern in the cytoplasm of a subset of cells (apparently cytotoxic lymphocytes) in the tested tonsil tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Granzyme B Antibody [NBP2-59678] - An intracellular stain was performed on Raw264.7 cells with NBP2-59678 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Granzyme B Antibody [NBP2-59678] - An intracellular stain was performed on U937 cells with NBP2-59678 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.