Explore
Validate
LearnABIN108719
antibody from antibodies-online
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
ELISA
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN108719 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN108719, RRID:AB_10953772
- Product name
- anti-Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated serine Esterase 1) (Gzmb) antibody
- Antibody type
- Monoclonal
- Antigen
- Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated serine Esterase 1) (Gzmb)
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
GM4C1 was generated by genetic immu
nisation of BALB/c mice with an imm
unisation vector containing the gra
nzyme B cDNA. It was purified by af
finity chromatography from cell cul
ture supernatants and is routinely
tested by flow cytometry on BOSC ce
lls trans- Isotype
- IgG
- Vial size
- 100 ug
Submitted references Granzyme B: pro-apoptotic, antiviral and antitumor functions.
Granzymes: a family of lymphocyte granule serine proteases.
Perforin/granzyme-dependent and independent mechanisms are both important for the development of graft-versus-host disease after murine bone marrow transplantation.
Trapani JA, Sutton VR
Current opinion in immunology 2003 Oct;15(5):533-43
Current opinion in immunology 2003 Oct;15(5):533-43
Granzymes: a family of lymphocyte granule serine proteases.
Trapani JA
Genome biology 2001;2(12):REVIEWS3014
Genome biology 2001;2(12):REVIEWS3014
Perforin/granzyme-dependent and independent mechanisms are both important for the development of graft-versus-host disease after murine bone marrow transplantation.
Graubert TA, DiPersio JF, Russell JH, Ley TJ
The Journal of clinical investigation 1997 Aug 15;100(4):904-11
The Journal of clinical investigation 1997 Aug 15;100(4):904-11
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using C1. BOSC23 cells were transiently trans-fected with an expression vector encoding either Granzyme B (red curve) or an irrelevant protein (control transfectant: black curve). Binding of C1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with Granzyme B trans-fected cells.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- BOSC cells were transiently transfected with expression vectors for Granzyme A, B, K, or M. Expression of the constructs was tested with an anti-tag monoclonal antibody (green curves), an irrelevant monoclonal antibody served as negative control (black curves). For specificity testing, purified C1 was tested on all transfectants. A positive signal was obtained only with Granzyme B transfected cells (red curves).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Intracellular detection of granzyme B in human PBMC by FACS analysis using C1. PBMC were cultivated in the presence of phorbolester and ionomycin subsequently fixed and permeabilised. Binding of C1 was detected with a FITC-conjugated secondary antibody.
