14-8822-37

antibody from Invitrogen Antibodies
Targeting: GZMB CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Provider product page for 14-8822-37
Western blot
ELISA
Immunohistochemistry
Other assay

Antibody data

Product number
14-8822-37 - Provider product page
Provider
Invitrogen Antibodies
Product name
Granzyme B Monoclonal Antibody (16G6), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 16G6 antibody reacts with mouse Granzyme B (GrB) which is a member of the granzyme serine protease family. GrB is found in the granules of cytotoxic T cells and NK cells. Granzyme B has also been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation. GrB has been called CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. GrB is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for granzyme B during cytotoxic T cell-induced apoptosis. For intracellular staining and flow cytometric analysis with direct conjugates of anti-mouse Granzyme B, it is highly recommended to use the Foxp3 Staining Buffer Set (Product # 00-5523). Other buffers may yield varying results. Applications Reported: This 16G6 antibody has been reported for use in western blotting, immunohistochemical staining, ELISA capture antibody, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: The unlabelled 16G6 antibody has been tested as the capture antibody in a sandwich ELISA for quantitation of mouse granzyme B protein levels, in combination with the biotinylated eBioLUEE (Product # 13-8821-81) antibody for detection and recombinant mouse granzyme B as the standard. A suitable range of concentrations of this antibody for ELISA detection is 1-4 µg/mL. The 16G6 antibody has been tested for Western blotting; a recommended starting concentration is 1 µg/mL. The 16G6 antibody has been used for formalin-fixed paraffin embedded tissue using low pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Host
Rat
Isotype
IgG
Antibody clone number
16G6
Vial size
2 mg
Concentration
0.5 mg/mL
Storage
4° C
GZMB protein structure - 14-8822-37 shown in red.