NBP1-97525

Liao P, Song K, Zhu Z, Liu Z, Zhang W, Li W, Hu J, Hu Q, Chen C, Chen B, McLeod HL, Pei H, Chen L, He Y
Clinical pharmacology and therapeutics 2020 May 17;

Deep immune profiling by mass cytometry links human T and NK cell differentiation and cytotoxic molecule expression patterns.

Bengsch B, Ohtani T, Herati RS, Bovenschen N, Chang KM, Wherry EJ
Journal of immunological methods 2018 Feb;453:3-10

Kv1.3 Channels Mark Functionally Competent CD8+ Tumor-Infiltrating Lymphocytes in Head and Neck Cancer.

Chimote AA, Hajdu P, Sfyris AM, Gleich BN, Wise-Draper T, Casper KA, Conforti L
Cancer research 2017 Jan 1;77(1):53-61

Granzyme-mediated cytotoxicity does not involve the mannose 6-phosphate receptors on target cells.

Dressel R, Raja SM, Höning S, Seidler T, Froelich CJ, von Figura K, Günther E
The Journal of biological chemistry 2004 May 7;279(19):20200-10

Acquisition of granzyme B and Fas ligand proteins by human keratinocytes contributes to epidermal cell defense.

Berthou C, Michel L, Soulié A, Jean-Louis F, Flageul B, Dubertret L, Sigaux F, Zhang Y, Sasportes M
Journal of immunology (Baltimore, Md. : 1950) 1997 Dec 1;159(11):5293-300

Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long-term surviving lung transplanted patients.

Berthou C, Legros-Maïda S, Soulié A, Wargnier A, Guillet J, Lafaurie C, Stern M, Sasportes M, Israël-Biet D
Transplantation proceedings 1996 Jun;28(3):1964-7

Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor.

Berthou C, Marolleau JP, Lafaurie C, Soulié A, Dal Cortivo L, Bourge JF, Benbunan M, Sasportes M
Blood 1995 Nov 1;86(9):3500-6

Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation.

Legros-Maïda S, Soulié A, Benvenuti C, Wargnier A, Vallée N, Berthou C, Guillet J, Sasportes M, Sigaux N
European journal of immunology 1994 Jan;24(1):229-33

Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients.

Clément MV, Legros-Maïda S, Israël-Biet D, Carnot F, Soulié A, Reynaud P, Guillet J, Gandjbakch I, Sasportes M
Transplantation 1994 Feb;57(3):322-6

Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients.

Clément MV, Legros-Maïda S, Israël-Biet D, Carnot F, Soulié A, Reynaud P, Guillet J, Gandjbakch I, Sasportes M
Transplantation 1994 Feb;57(3):322-6

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Granzyme B Antibody (B18.1) [NBP1-97525] - Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species. Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: Granzyme B Antibody (B18.1) [NBP1-97525] - An intracellular stain was performed on U937 cells with Granzyme B [B18.1] Antibody NBP1-97525AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: Granzyme B Antibody (B18.1) [NBP1-97525] - [FITC] Detection of granzyme B in permeabilized human CD8+ T lymphocytes (filled profile). Isotype control in combination with a secondary FITC-labeled antibody is shown as control. Method: Permeabilized human T cells (5 x 10^5).