NBP1-88151
antibody from Novus Biologicals
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [7]
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Validation data
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- Product number
- NBP1-88151 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88151, RRID:AB_11011354
- Product name
- Rabbit Polyclonal Granzyme B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Granzyme B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Exploiting antitumor immunity to overcome relapse and improve remission duration.
Chen LL, Chen X, Choi H, Sang H, Chen LC, Zhang H, Gouw L, Andtbacka RH, Chan BK, Rodesch CK, Jimenez A, Cano P, Jones KA, Oyedeji CO, Martins T, Hill HR, Schumacher J, Willmore C, Scaife CL, Ward JH, Morton K, Randall RL, Lazar AJ, Patel S, Trent JC, Frazier ML, Lin P, Jensen P, Benjamin RS
Cancer immunology, immunotherapy : CII 2012 Jul;61(7):1113-24
Cancer immunology, immunotherapy : CII 2012 Jul;61(7):1113-24
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Granzyme B Antibody [NBP1-88151] - Analysis in control (vector only transfected HEK293T lysate) and GZMB over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human lymph node shows strong cytoplasmic positivity in a subset of lymphoid cells outside reaction center.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human cerebral cortex shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining in human lymph node and skeletal muscle tissues using NBP1-88151 antibody. Corresponding GZMB RNA-seq data are presented for the same tissues.