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LearnPA5-17457
antibody from Invitrogen Antibodies
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- PA5-17457 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Granzyme B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references DNA-PK-mediated phosphorylation of EZH2 regulates the DNA damage-induced apoptosis to maintain T-cell genomic integrity.
Wang Y, Sun H, Wang J, Wang H, Meng L, Xu C, Jin M, Wang B, Zhang Y, Zhang Y, Zhu T
Cell death & disease 2016 Jul 28;7(7):e2316
Cell death & disease 2016 Jul 28;7(7):e2316
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of NK-92 (Lane 1) and NK-92 treated with IL-2 (100 ng/mL for 48 hours) (Lane 2). The blot was probed with Anti-Granzyme B Polyclonal Antibody (Product # PA5-17457, 1:2500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 32 kDa band corresponding to Granzyme B was observed in the cell line tested and was enhanced upon treatment.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Granzyme B in recombinant human Granzyme B (5 ng) and extracts from the natural killer cell line NK-92 using Granzyme B polyclonal antibody (Product # PA5-17457).
