ALX-804-121-C100
antibody from Enzo Life Sciences
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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- Product number
- ALX-804-121-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-121-C100, RRID:AB_2051556
- Product name
- Granzyme B (human) monoclonal antibody (B18.1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- B18.1
- Vial size
- 100 μg
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Granzyme-mediated cytotoxicity does not involve the mannose 6-phosphate receptors on target cells.
Acquisition of granzyme B and Fas ligand proteins by human keratinocytes contributes to epidermal cell defense.
Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long-term surviving lung transplanted patients.
Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor.
Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation.
Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients.
Dressel R, Raja SM, Höning S, Seidler T, Froelich CJ, von Figura K, Günther E
The Journal of biological chemistry 2004 May 7;279(19):20200-10
The Journal of biological chemistry 2004 May 7;279(19):20200-10
Acquisition of granzyme B and Fas ligand proteins by human keratinocytes contributes to epidermal cell defense.
Berthou C, Michel L, Soulié A, Jean-Louis F, Flageul B, Dubertret L, Sigaux F, Zhang Y, Sasportes M
Journal of immunology (Baltimore, Md. : 1950) 1997 Dec 1;159(11):5293-300
Journal of immunology (Baltimore, Md. : 1950) 1997 Dec 1;159(11):5293-300
Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long-term surviving lung transplanted patients.
Berthou C, Legros-Maïda S, Soulié A, Wargnier A, Guillet J, Lafaurie C, Stern M, Sasportes M, Israël-Biet D
Transplantation proceedings 1996 Jun;28(3):1964-7
Transplantation proceedings 1996 Jun;28(3):1964-7
Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor.
Berthou C, Marolleau JP, Lafaurie C, Soulié A, Dal Cortivo L, Bourge JF, Benbunan M, Sasportes M
Blood 1995 Nov 1;86(9):3500-6
Blood 1995 Nov 1;86(9):3500-6
Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation.
Legros-Maïda S, Soulié A, Benvenuti C, Wargnier A, Vallée N, Berthou C, Guillet J, Sasportes M, Sigaux N
European journal of immunology 1994 Jan;24(1):229-33
European journal of immunology 1994 Jan;24(1):229-33
Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients.
Clément MV, Legros-Maïda S, Israël-Biet D, Carnot F, Soulié A, Reynaud P, Guillet J, Gandjbakch I, Sasportes M
Transplantation 1994 Feb;57(3):322-6
Transplantation 1994 Feb;57(3):322-6
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species. Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Detection of granzyme B in a human CD8+ T cell clone. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 µg/ml of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.